Prevalence and comparison of genetic profiles of Campylobacter strains isolated from poultry and sporadic cases of campylobacteriosis in humans.

Between July 1998 and June 1999, 93 lots of broiler chickens distributed on 57 farms were sampled in two abattoirs of the province of Quebec (Canada). A total of 2,325 samples of cecal material were analyzed to determine the prevalence of campylobacters. Biotyping and pulsed-field gel electrophoresis (PFGE) were done on 20% of the Campylobacter isolates to study the distribution within poultry production. Macrorestriction profiles were compared with profiles of 24 Campylobacter strains isolated from sporadic cases of human diarrheic patients in order to evaluate genetic relationships. Approximately 40% of the broiler chickens in 60% of the lots and 67% of the farms were colonized. Biotypes I and II of Campylobacter jejuni were the most prevalent biotypes in poultry and human isolates. The PFGE dendograms revealed a high genetic diversity among poultry isolates, with 49 different genotypes from the 56 positive lots. More than 75% of these lots were colonized by a unique genotype. All positive lots raised simultaneously on the same farm had common genotype(s). Different genotypes were isolated from lots raised at different grow-out periods on a farm. In some cases, identical genotypes were found at different grow-out periods on a farm and also from different farms. Macrorestriction profiles showed that approximately 20% of human Campylobacter isolates were genetically related to genotypes found in poultry. This genetic relationship and the high prevalence of C. jejuni biotypes I and II in poultry indicated that Campylobacter in broiler production of the province of Quebec could be a potential source of hazard for public health.     

Strategies for combining decision procedures

Implementing efficient algorithms for combining decision procedures has been a challenge and their correctness precarious. In this paper we describe an inference system that has the classical Nelson–Oppen procedure at its core and includes several optimizations: variable abstraction with sharing, canonization of terms at the theory level, and Shostak's streamlined generation of new equalities for theories with solvers. The transitions of our system are fine-grained enough to model most of the mechanisms currently used in designing combination procedures. In particular, with a simple language of regular expressions we are able to describe several combination algorithms as strategies for our inference system, from the basic Nelson–Oppen to the very highly optimized one recently given by Shankar and Rueß. Presenting the basic system at a high level of generality and non-determinism allows transparent correctness proofs that can be extended in a modular fashion when new features are introduced in the system.     

A method to predict multipath effects on a line-of-sight link

A method that computes the quality of a digital microwave line-of-sight link affected by multipath propagation is presented. The use of a statistical propagation model and of the signature concept allows the propagation effects to be separated from the characteristics of the equipment. Since the variations of the statistical mode coefficients with respect to the characteristics of the link under consideration are not known, experimental results have been achieved using assumptions based on physical considerations. A single channel is considered and the same principles, although more speculative because of the lack of data, are applied to a diversity channel     

Accelerated Aging in 3-mol%-Yttria-Stabilized Tetragonal Zirconia Ceramics Sintered in Reducing Conditions

The aging behavior of 3-mol%-yttria-stabilized tetragonal zirconia (3Y-TZP) ceramics sintered in air and in reducing conditions was investigated at 140°C in water vapor. It was observed by X-ray diffraction (XRD) that 3Y-TZP samples sintered in reducing conditions exhibited significantly higher tetragonal-to-monoclinic transformation than samples with similar density and average grain size values but obtained by sintering in air. This fact is explained by the increase of the oxygen vacancy concentration and by the presence at the grain boundary region of a new aggregate phase formed because of the exolution of Fe²⁺ ions observed by X-ray photoelectron spectroscopy.     

A 3D EFFECT IN THE WEDGE ADHESION TEST: APPLICATION OF SPECKLE INTERFEROMETRY

The wedge test is of considerable use for evaluating adhesion between two bonded rigid substrates. In its (usual) static form, release of elastic strain energy is equated to effective adhesion energy during crack growth. However, the test is usually treated as two-dimensional. In fact, it is really three-dimensional due to anticlastic bending effects of the bent beam(s) during crack propagation.

We studied a composite material/epoxy/aluminium alloy system and observed a curved crack front during propagation. This leads to doubt as to the value of crack length to be inserted in the adhesion energy formula. In addition, by using the highly sensitive technique of speckle interferometry, it was possible to study anticlastic bending effects in a quantitative manner. Far from the crack front, agreement between theory and experimental is good, yet work remains to be done to understand the zone near the fracture zone.     

Latent risk and trend models for the evolution of annual fatality numbers in 30 European countries

In this paper a unified methodology is presented for the modelling of the evolution of road safety in 30 European countries. For each country, annual data of the best available exposure indicator and of the number of fatalities were simultaneously analysed with the bivariate latent risk time series model. This model is based on the assumption that the amount of exposure and the number of fatalities are intrinsically related. It captures the dynamic evolution in the fatalities as the product of the dynamic evolution in two latent trends: the trend in the fatality risk and the trend in the exposure to that risk. Before applying the latent risk model to the different countries it was first investigated and tested whether the exposure indicator at hand and the fatalities in each country were in fact related at all. If they were, the latent risk model was applied to that country; if not, a univariate local linear trend model was applied to the fatalities series only, unless the latent risk time series model was found to yield better forecasts than the univariate local linear trend model. In either case, the temporal structure of the unobserved components of the optimal model was established, and structural breaks in the trends related to external events were identified and captured by adding intervention variables to the appropriate components of the model. As a final step, for each country the optimally modelled developments were projected into the future, thus yielding forecasts for the number of fatalities up to and including 2020.     

Coplanar perturbation of a crack lying on the mid-plane of a plate

Several groups have studied experimentally the deformation of the front of mode I cracks propagating quasistatically along the interface between bonded plates. The theoretical interpretation of such experiments has always been based up to now on a formula of Rice (ASME J Appl Mech 52:571–579, 1985); this formula provides the first-order variation of the local mode I stress intensity factor resulting from some small, but otherwise arbitrary coplanar perturbation of the front of a semi-infinite crack in an infinite body. To be applicable to bonded plates, this formula requires that the characteristic distance of variation of this perturbation in the direction of the crack front be small compared to all other characteristic dimensions of the problem, and first of all the thickness of the plates. This condition is unfortunately frequently violated in practice. The purpose of this paper is therefore to provide a more exact formula for the variation of the local stress intensity factor, for the specific cracked geometry and boundary conditions used in experiments; this should allow for more accurate theoretical interpretations. This is done in two steps. The first one consists in adapting Rice’s (ASME J Appl Mech 52:571–579, 1985) treatment, applicable to the extreme case of plates of infinite thickness, to the other extreme one of plates of infinitesimal thickness, using the standard Love-Kirchhoff plate theory. An interesting outcome of the analysis is that the distance from the crack front to the boundary of the plate acts as a “cutoff length”, in the sense that when the distance between two points on the crack front gets larger than it, the influence of the crack advance at the first point upon the stress intensity factor at the second diminishes quickly; the plate thickness, however, plays no similar role. The second step consists in supplementing the theoretical expressions applicable to extreme values of the plate thickness with finite element computations providing results for intermediate values. These computations lead to the definition of a simple, approximate but accurate “interpolation formula” for the variation of the local stress intensity factor, applicable to plates of arbitrary thickness.     

Antimicrobial effect of cranberry juice and extracts

The antimicrobial effect of cranberry juice and of three cranberry extracts (water-soluble (E1) and apolar phenolic compounds (E2), and anthocyanins (E3)) was investigated against seven bacterial strains (Enterococcus faecium resistant to vancomycin (ERV), Escherichia coli O157:H7 EDL 933, Escherichia coli ATCC 25922, Listeria monocytogenes HPB 2812, Pseudomonas aeruginosa ATCC 15442, Salmonella Typhimurium SL1344, and Staphylococcus aureus ATCC 29213). Each cranberry sample was analyzed to determine the minimum inhibitory concentration (MIC) and the maximal tolerated concentration (MTC) at neutral pH. The results, reported in μg phenol/mL, indicated that all the bacterial strains, both Gram-positive and Gram-negative, were selectively inhibited by the cranberry phenolic compounds. The extract rich in water-soluble phenolic compounds caused the most important growth inhibitions. The bacteria ERV, and to a lesser degree, P. aeruginosa, S. aureus and E. coli ATCC 25922, were the most sensitive to the antimicrobial activity of extract E1. The growth of P. aeruginosa and E. coli ATCC was also affected by the presence of the anthocyanin-rich cranberry extract E3, although the observed antibacterial effect was not as important as with extract E1. In general, L. monocytogenes, E. coli O157:H7 and S. Typhimurium were the most resistant to the antibacterial activity of the cranberry extracts. Within 30 min of exposure with pure neutralized cranberry juice, L. monocytogenes and ERV were completely inactivated.     

Carbon and sulfur cycling by microbial communities in a gypsum-treated oil sands tailings pond

Oil sands tailings ponds receive and store the solid and liquid waste from bitumen extraction and are managed to promote solids densification and water recycling. The ponds are highly stratified due to increasing solids content as a function of depth but can be impacted by tailings addition and removal and by convection due to microbial gas production. We characterized the microbial communities in relation to microbial activities as a function of depth in an active tailings pond routinely treated with gypsum (CaSO(4)·2H(2)O) to accelerate densification. Pyrosequencing of 16S rDNA gene sequences indicated that the aerobic surface layer, where the highest level of sulfate (6 mM) but no sulfide was detected, had a very different community profile than the rest of the pond. Deeper anaerobic layers were dominated by syntrophs (Pelotomaculum, Syntrophus, and Smithella spp.), sulfate- and sulfur-reducing bacteria (SRB, Desulfocapsa and Desulfurivibrio spp.), acetate- and H(2)-using methanogens, and a variety of other anaerobes that have been implicated in hydrocarbon utilization or iron and sulfur cycling. The SRB were most abundant from 10 to 14 mbs, bracketing the zone where the sulfate reduction rate was highest. Similarly, the most abundant methanogens and syntrophs identified as a function of depth closely mirrored the fluctuating methanogenesis rates. Methanogenesis was inhibited in laboratory incubations by nearly 50% when sulfate was supplied at pond-level concentrations suggesting that in situ sulfate reduction can substantially minimize methane emissions. Based on our data, we hypothesize that the emission of sulfide due to SRB activity in the gypsum treated pond is also limited due to its high solubility and oxidation in surface waters.     

Multipath optimized link state routing for mobile ad hoc networks

Multipath routing protocols for Mobile Ad hoc NETwork (MANET) address the problem of scalability, security (confidentiality and integrity), lifetime of networks, instability of wireless transmissions, and their adaptation to applications.Our protocol, called MultiPath OLSR (MP-OLSR), is a multipath routing protocol based on OLSR [1]. The Multipath Dijkstra Algorithm is proposed to obtain multiple paths. The algorithm gains great flexibility and extensibility by employing different link metrics and cost functions. In addition, route recovery and loop detection are implemented in MP-OLSR in order to improve quality of service regarding OLSR. The backward compatibility with OLSR based on IP source routing is also studied. Simulation based on Qualnet simulator is performed in different scenarios. A testbed is also set up to validate the protocol in real world. The results reveal that MP-OLSR is suitable for mobile, large and dense networks with large traffic, and could satisfy critical multimedia applications with high on time constraints.